| Gene | Spsb2 |
| Chromosome | 6 |
| Deletion Start | 124,809,309 |
| Deletion End | 124,810,405 |
| Deletion Size | 1,097 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,809,649 | 124,809,667 | TGCAGGCTGACCACTATGC |
| TUP | 124,809,675 | 124,809,695 | TTGGGCAGCAACAGCGAGTCC |
| TUR | 124,809,729 | 124,809,748 | TCGAGGCCCTTACTCTGATG |
| TDF | 124,809,901 | 124,809,925 | TCTATCCCTCTGTAAGTGCTGTTTG |
| TDP | 124,809,931 | 124,809,952 | AGTGCCAGGTCCGCATCCGCTA |
| TDR | 124,809,967 | 124,809,988 | CACCTAGTCCGTATCTCACCTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGCTCCTGAGAAACTTGG |
| SD | ATTCCTTAAGACAGTGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11811A-A5 | VGB6 | C57BL/6NTac | |
| 11811A-A9 | VGB6 | C57BL/6NTac | |
| 11811A-E3 | VGB6 | C57BL/6NTac | |
| 11811A-C12 | VGB6 | C57BL/6NTac | |
