| Gene | Nrl |
| Chromosome | 14 |
| Deletion Start | 55,523,437 |
| Deletion End | 55,521,478 |
| Deletion Size | 1,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,522,335 | 55,522,355 | GTTGGGCTCCACACCATACAG |
| TUP | 55,522,310 | 55,522,332 | CGGTGCCTCCTTCACCCACCTTC |
| TUR | 55,522,241 | 55,522,261 | GGCCAGCCAATATAGCTCCTC |
| TDF | 55,521,037 | 55,521,059 | CATGGTCCAGGTGTGATAGAATC |
| TDP | 55,521,002 | 55,521,031 | TGAGGTTTGCAGGAAGAACAGTATACAGCA |
| TDR | 55,520,976 | 55,520,994 | AAAGCCCTCTAGGCTCCAC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGGAAGTAGCTGTTCAG |
| SD | CATTCAGCATGCCACCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11812A-H9 | VGB6 | C57BL/6NTac | |
| 11812A-C5 | VGB6 | C57BL/6NTac | |
| 11812A-G4 | VGB6 | C57BL/6NTac | |
| 11812A-E9 | VGB6 | C57BL/6NTac | |
