| Gene | Gja3 |
| Chromosome | 14 |
| Deletion Start | 57,058,066 |
| Deletion End | 57,056,695 |
| Deletion Size | 1,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,036,866 | 57,036,884 | GCTGGAGAACGCACAGGAG |
| TUP | 57,036,838 | 57,036,862 | TCTACAGTCATCGGCAAAGTGTGGC |
| TUR | 57,036,806 | 57,036,827 | CACCAGAATGCGGAAGATGAAC |
| TDF | 57,035,869 | 57,035,888 | AGCCAGCAAGCCCTCTTCAG |
| TDP | 57,035,843 | 57,035,865 | CATCCAGCCCTGATGGCCGCAAG |
| TDR | 57,035,789 | 57,035,807 | CGTCACTACCAAGGCACTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTGTTAGCACAGCTTTACC |
| SD | CTTGGAGAGCTTAGGAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11814A-B3 | VGB6 | C57BL/6NTac | |
| 11814A-G11 | VGB6 | C57BL/6NTac | |
| 11814A-E7 | VGB6 | C57BL/6NTac | |
| 11814A-D5 | VGB6 | C57BL/6NTac | |
| 11814A-F1 | VGB6 | C57BL/6NTac | |
