| Gene | Tmem150a |
| Chromosome | 6 |
| Deletion Start | 72,356,251 |
| Deletion End | 72,359,283 |
| Deletion Size | 3,033 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,356,750 | 72,356,767 | GTGTGCCCAGTGGAGAAC |
| TUP | 72,356,768 | 72,356,793 | TGGTATGGAGCACTCTACAGATGGCC |
| TUR | 72,356,808 | 72,356,829 | CAAGGAGGGACAGAGATGAAGG |
| TDF | 72,358,850 | 72,358,871 | CCACTGCCCTAGTCCTAGTCTG |
| TDP | 72,358,872 | 72,358,894 | ATGCGGCCCGGTCCTTTACCTCT |
| TDR | 72,358,896 | 72,358,915 | AGGCCATCCCAAGCAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCCAGATGGATAAGCCAGAG |
| SD | ACTCGTGTGGTTCTGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11815A-B1 | VGB6 | C57BL/6NTac | |
| 11815A-B11 | VGB6 | C57BL/6NTac | |
| 11815A-C7 | VGB6 | C57BL/6NTac | |
| 11815A-D5 | VGB6 | C57BL/6NTac | |
