| Gene | Tmem111 |
| Chromosome | 6 |
| Deletion Start | 113,531,479 |
| Deletion End | 113,515,882 |
| Deletion Size | 15,598 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,530,830 | 113,530,851 | GCTTGGAGACTACCACAGAGAC |
| TUP | 113,530,803 | 113,530,828 | TGTGAGGAGTTGCGATGGACCATTCC |
| TUR | 113,530,770 | 113,530,793 | CTGTAAGAAGGCAAGTGAGGAGAG |
| TDF | 113,516,045 | 113,516,065 | CCTCTTAGCATCCCTGTGTTC |
| TDP | 113,516,021 | 113,516,044 | TCTTCCTGTGCAGACAGAGTGGGA |
| TDR | 113,515,990 | 113,516,008 | CGCCCACTGGTGATCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TAGAACAAGTCAGGGCCGTC |
| SD | TACTGTGGCTGGAAGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11816A-C10 | VGB6 | C57BL/6NTac | |
| 11816A-G6 | VGB6 | C57BL/6NTac | |
| 11816A-D5 | VGB6 | C57BL/6NTac | |
| 11816A-D2 | VGB6 | C57BL/6NTac | |
