| Gene | Cops7b |
| Chromosome | 1 |
| Deletion Start | 86,589,183 |
| Deletion End | 86,605,292 |
| Deletion Size | 16,110 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,589,984 | 86,590,006 | TGTGGTTGGCATCTGTTAGGTAG |
| TUP | 86,590,015 | 86,590,039 | CATGGCCACAATGACACGATTGCCA |
| TUR | 86,590,066 | 86,590,087 | ATAGCCTGGACCCACAGCTCAG |
| TDF | 86,604,965 | 86,604,982 | GAGGATTTGGGCCGCTTC |
| TDP | 86,604,983 | 86,605,012 | TCTGGGTGGTCCTTATACTTAGAACAGGAC |
| TDR | 86,605,044 | 86,605,064 | TGCTAACCTGGAGGGAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGTCAGCTTTATGGAC |
| SD | TAAACTGCCTCCAGCTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11821A-C9 | VGB6 | C57BL/6NTac | |
| 11821A-F1 | VGB6 | C57BL/6NTac | |
| 11821A-A3 | VGB6 | C57BL/6NTac | |
