| Gene | Tmem79 |
| Chromosome | 3 |
| Deletion Start | 88,333,826 |
| Deletion End | 88,329,871 |
| Deletion Size | 3,956 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,333,060 | 88,333,079 | AAGCTGTGGAGGTCGTGAGG |
| TUP | 88,333,037 | 88,333,059 | CACTGAGAGCTGTCGCCTCGGTG |
| TUR | 88,332,974 | 88,332,996 | ATCGAAAGGCAGGAAGGCGTATG |
| TDF | 88,329,857 | 88,329,875 | CCTGACTTTCCTGCCACTC |
| TDP | 88,329,834 | 88,329,856 | TTGGCCATGCTGGTGTGGAACCT |
| TDR | 88,329,769 | 88,329,787 | GGTAGTCCAAGCGGCTCTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTGTCCTGCTGGATGAC |
| SD | TAGCACCTGTGCCATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11824A-F6 | VGB6 | C57BL/6NTac | |
| 11824A-G4 | VGB6 | C57BL/6NTac | |
