| Gene | 1700011F03Rik |
| Chromosome | 6 |
| Deletion Start | 70,918,783 |
| Deletion End | 70,914,320 |
| Deletion Size | 4,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,917,222 | 70,917,242 | TCTAGCTCAGTGTCTGGGAAC |
| TUP | 70,917,193 | 70,917,220 | TCTAATCCTCTGCCTGCTATCCTAGGCC |
| TUR | 70,917,146 | 70,917,169 | GCCATGTGAGATTTAGGGTAAGTG |
| TDF | 70,914,093 | 70,914,112 | TGCATGAGGACCTGGGTTTG |
| TDP | 70,914,058 | 70,914,084 | AACCCACAGGCACAGTAGCATGTCTCA |
| TDR | 70,914,030 | 70,914,051 | CCTGTCTGTCTCCCTAGCACTG |
PCR Primers
| Primer | Sequence |
| SU | AACGGATCTGATATTTTGAGTG |
| SD | CTACATACATTCTATACACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11827A-A1 | VGB6 | C57BL/6NTac | |
| 11827A-A12 | VGB6 | C57BL/6NTac | |
| 11827A-E3 | VGB6 | C57BL/6NTac | |
| 11827A-D3 | VGB6 | C57BL/6NTac | |
| 11827A-C6 | VGB6 | C57BL/6NTac | |
| 11827A-F3 | VGB6 | C57BL/6NTac | |
