| Gene | Alas2 |
| Chromosome | X |
| Deletion Start | 150,552,167 |
| Deletion End | 150,570,551 |
| Deletion Size | 18,385 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 150,552,289 | 150,552,311 | CACTCAAGGACCAACCTGTTCTC |
| TUP | 150,552,315 | 150,552,340 | TCCATCTTAAGGCAACCAAGGCTGGA |
| TUR | 150,552,349 | 150,552,371 | TCCCATTTGCCAATAACCTCTTC |
| TDF | 150,570,391 | 150,570,411 | GCAACTTCTGTCATCGTCCTG |
| TDP | 150,570,416 | 150,570,443 | CTTTGAACTTATGAGCGAGTGGGAGCGA |
| TDR | 150,570,445 | 150,570,466 | GGTCCCATGTTCCCAAAGTAGG |
PCR Primers
| Primer | Sequence |
| SU | GGCATCTGTCCACATAAGTG |
| SD | TTTGTACAGCTTCCAGTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11828B-A2 | VGB6 | C57BL/6NTac | |
| 11828B-E2 | VGB6 | C57BL/6NTac | |
