| Gene | Rrp8 |
| Chromosome | 7 |
| Deletion Start | 105,736,393 |
| Deletion End | 105,732,938 |
| Deletion Size | 3,456 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,735,798 | 105,735,818 | GACAAGCAGCAGGACTTGAAC |
| TUP | 105,735,771 | 105,735,796 | CCAAATCAGCTTCACGGAAAGCAGGC |
| TUR | 105,735,686 | 105,735,705 | GCAGAACATCCTGGGCTAAC |
| TDF | 105,733,306 | 105,733,328 | GCCCAGTTCTTAACCTCTTTCAG |
| TDP | 105,733,273 | 105,733,296 | TTCAGGAGGGAGGCTGTAAACCCA |
| TDR | 105,733,231 | 105,733,248 | CCCTCCCAGAGCTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCAGAGATCGCAGAGAC |
| SD | AGTGGCAAACTAGCATTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11836A-A2 | VGB6 | C57BL/6NTac | |
| 11836A-A12 | VGB6 | C57BL/6NTac | |
| 11836A-B1 | VGB6 | C57BL/6NTac | |
| 11836A-H2 | VGB6 | C57BL/6NTac | |
