| Gene | Lrat |
| Chromosome | 3 |
| Deletion Start | 82,899,588 |
| Deletion End | 82,892,850 |
| Deletion Size | 6,739 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,903,428 | 82,903,449 | TCAGAAGGTGGTCTCCAACAAG |
| TUP | 82,903,403 | 82,903,427 | CGTCTCCTCCTGGGAGTCATTTGCA |
| TUR | 82,903,351 | 82,903,370 | TCTGCTCCGTAGGCAAAGTC |
| TDF | 82,897,820 | 82,897,844 | TCCATTACAGATGGTTGTGAGCTAC |
| TDP | 10,167,205 | 10,167,229 | TGGCTGCTGGGAATTGAACTCAGGA |
| TDR | 82,897,765 | 82,897,784 | AATCGCACTGACGGCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TACCTCTTCAGCTCCTCAAG |
| SD | GACTTCTGACAGGCTGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11842A-F2 | VGB6 | C57BL/6NTac | |
| 11842A-F10 | VGB6 | C57BL/6NTac | |
| 11842A-F11 | VGB6 | C57BL/6NTac | |
