| Gene | Gjb5 |
| Chromosome | 4 |
| Deletion Start | 127,357,799 |
| Deletion End | 127,356,627 |
| Deletion Size | 1,173 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,356,305 | 127,356,324 | TCCTGAGCGGAGTCAACAAG |
| TUP | 127,356,281 | 127,356,303 | ACTCCACAGCCTTTGGTCGCATC |
| TUR | 127,356,236 | 127,356,255 | CACCAGGTACACCAGCACAC |
| TDF | 127,355,660 | 127,355,682 | CACATGCAAAGAATGACCCAAAC |
| TDP | 127,355,635 | 127,355,659 | TGGGCCAACTCTCCTAGCAAAGAGA |
| TDR | 127,355,593 | 127,355,612 | CGTCCGAGCCCAGAAAGATG |
PCR Primers
| Primer | Sequence |
| SU | CAGCAAAGAACAGAAAGTAGG |
| SD | AGCCACGCTTACTCAGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11843A-A10 | VGB6 | C57BL/6NTac | |
| 11843A-B9 | VGB6 | C57BL/6NTac | |
| 11843A-C8 | VGB6 | C57BL/6NTac | |
| 11843A-E11 | VGB6 | C57BL/6NTac | |
| 11843A-H7 | VGB6 | C57BL/6NTac | |
| 11843A-A12 | VGB6 | C57BL/6NTac | |
