11845 Gltpd2 Status: ES cell colonies screened / QC no positives
| Gene | Gltpd2 |
| Chromosome | 11 |
| Deletion Start | 70,519,221 |
| Deletion End | 70,520,519 |
| Deletion Size | 1,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,519,620 | 70,519,641 | CAGCTAAACCTGAGGGACATTC |
| TUP | 70,519,643 | 70,519,666 | TCAGGGCCCAACAGTGGCTAAGTA |
| TUR | 70,519,706 | 70,519,724 | GGGCCAGTCTGGGAGATAG |
| TDF | 70,519,994 | 70,520,012 | TCTTCGCCTTTGCCACCAG |
| TDP | 70,520,013 | 70,520,036 | CGAGGCCTTCAACAAGGTGACCGA |
| TDR | 70,520,069 | 70,520,090 | GTCATCAGCGATGTGTAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTCTTACGACAAGCTAG |
| SD | ATACATAACGCCGGATGTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
