| Gene | Rgs9bp |
| Chromosome | 7 |
| Deletion Start | 35,585,277 |
| Deletion End | 35,584,510 |
| Deletion Size | 768 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,585,169 | 35,585,188 | CGCGCTCAACAAGACCACAG |
| TUP | 35,585,139 | 35,585,168 | CGTGCTACCATCACTTAGTGCTTACTGTGG |
| TUR | 35,585,099 | 35,585,116 | TGCAGCTCTTCGCGTAGG |
| TDF | 35,584,737 | 35,584,756 | CCAGGTGGGCGAGATGATTG |
| TDP | 35,584,708 | 35,584,735 | CGACATGGAAATGAAAGTCAACGTGCCC |
| TDR | 35,584,624 | 35,584,642 | AATGCCACCGGCTGAGGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGGTGCAAAGACACTACG |
| SD | CACCAAACTTGGGACATACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11847A-A11 | VGB6 | C57BL/6NTac | |
| 11847A-B5 | VGB6 | C57BL/6NTac | |
| 11847A-C2 | VGB6 | C57BL/6NTac | |
| 11847A-D3 | VGB6 | C57BL/6NTac | |
| 11847A-H11 | VGB6 | C57BL/6NTac | |
| 11847A-H4 | VGB6 | C57BL/6NTac | |
