| Gene | Smad1 |
| Chromosome | 8 |
| Deletion Start | 79,372,180 |
| Deletion End | 79,339,582 |
| Deletion Size | 32,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,372,017 | 79,372,037 | AGAGCTGGAGAAGGCCCTGAG |
| TUP | 79,371,994 | 79,372,015 | TGCCCTGGACAGCCGAGTAACT |
| TUR | 79,371,967 | 79,371,986 | CATCCAGGGAGCGAGGAATG |
| TDF | 79,340,542 | 79,340,563 | GTGTCAGGCTCCATCATTCATC |
| TDP | 79,340,513 | 79,340,538 | TCAGGAAGGTGGCTTGCTACATGGTA |
| TDR | 79,340,461 | 79,340,481 | GGCTTTCATGGTGACCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | ACCGAGTTGTGAGGCTGAAC |
| SD | AAAGACTATCAGCGCCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11850A-D5 | VGB6 | C57BL/6NTac | |
| 11850A-H1 | VGB6 | C57BL/6NTac | |
| 11850B-H6 | VGB6 | C57BL/6NTac | |
