| Gene | Krcc1 |
| Chromosome | 6 |
| Deletion Start | 71,283,989 |
| Deletion End | 71,284,786 |
| Deletion Size | 798 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,284,140 | 71,284,163 | CCAGGCCCAGGTATAGAATGTTTG |
| TUP | 71,284,165 | 71,284,191 | TCAGAGACTCCCATCTGGAACCAACCA |
| TUR | 71,284,212 | 71,284,234 | CCGGTCTTCTGTCTGTGAACTAC |
| TDF | 71,284,693 | 71,284,715 | GGAACAAGGTGAAGAAAGGACAG |
| TDP | 71,284,716 | 71,284,745 | AGGAGGAGATGCTTTGGGACCAGTCTATTC |
| TDR | 71,284,769 | 71,284,786 | TCAGTGGAGCCTCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCCACAGACATGATTCAC |
| SD | CCACTACCACAGCAATTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11852A-C5 | VGB6 | C57BL/6NTac | |
| 11852A-C8 | VGB6 | C57BL/6NTac | |
| 11852A-C12 | VGB6 | C57BL/6NTac | |
| 11852A-D7 | VGB6 | C57BL/6NTac | |
| 11852A-F3 | VGB6 | C57BL/6NTac | |
| 11852A-F10 | VGB6 | C57BL/6NTac | |
| 11852A-G3 | VGB6 | C57BL/6NTac | |
