| Gene | Aldoa |
| Chromosome | 7 |
| Deletion Start | 126,800,270 |
| Deletion End | 126,797,930 |
| Deletion Size | 2,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,796,891 | 126,796,912 | GCAGGTAGATAAGGGTGTGGTG |
| TUP | 126,796,865 | 126,796,888 | CCTGGCAGGAACCAATGGCGAGAC |
| TUR | 126,796,831 | 126,796,849 | GGAGGCAGGCAGATCATTC |
| TDF | 126,796,128 | 126,796,147 | CACCTTTCCCTGCTGTGTTC |
| TDP | 126,796,106 | 126,796,127 | CAGGTCCTGGCGGCTGTCTACA |
| TDR | 126,796,082 | 126,796,100 | AGACATGGTGGTCGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CAGCAACAGACAGAGTTAGG |
| SD | TGCCTTTCTCAAAGCTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11857A-A1 | VGB6 | C57BL/6NTac | |
| 11857A-B6 | VGB6 | C57BL/6NTac | |
| 11857A-B9 | VGB6 | C57BL/6NTac | |
| 11857A-C6 | VGB6 | C57BL/6NTac | |
| 11857A-D9 | VGB6 | C57BL/6NTac | |
| 11857A-G11 | VGB6 | C57BL/6NTac | |
