| Gene | Atp5g1 |
| Chromosome | 11 |
| Deletion Start | 96,071,453 |
| Deletion End | 96,069,500 |
| Deletion Size | 1,954 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,074,574 | 96,074,593 | CCCAGGTGATGCCAATGTTC |
| TUP | 96,074,547 | 96,074,573 | TGCCAAGCCTGAGAGTCATTGTACTGA |
| TUR | 96,074,501 | 96,074,523 | CCATCTTAGGAAAGCTCCCAGTG |
| TDF | 96,073,569 | 96,073,588 | TGGTGTGGCTGGATCAGGAG |
| TDP | 96,073,543 | 96,073,568 | CTGGCATTGGCACAGTGTTTGGTAGC |
| TDR | 96,073,481 | 96,073,502 | CCAAGTGGAGTGGAGCAACAAC |
PCR Primers
| Primer | Sequence |
| SU | TCGGTTGCTGTCCTTCTGC |
| SD | AAGTGACAAAGCACCAACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11858A-D10 | VGB6 | C57BL/6NTac | |
| 11858A-F4 | VGB6 | C57BL/6NTac | |
| 11858A-F10 | VGB6 | C57BL/6NTac | |
| 11858A-F12 | VGB6 | C57BL/6NTac | |
