| Gene | Slc37a4 |
| Chromosome | 9 |
| Deletion Start | 44,398,477 |
| Deletion End | 44,402,468 |
| Deletion Size | 3,992 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,398,487 | 44,398,507 | GCTACGGCTACTATCGCACTG |
| TUP | 44,398,509 | 44,398,533 | CATATTTGCGGCCATGTTTGGAGGC |
| TUR | 44,398,563 | 44,398,584 | GGAGGGCATGACAAAGGAGAAG |
| TDF | 44,402,296 | 44,402,313 | CCGCAGTGGGTGGATTTC |
| TDP | 44,402,314 | 44,402,336 | TGGCTGGCTTACCCTTCAGCACC |
| TDR | 44,402,377 | 44,402,397 | TGGCTCCACAAACCACTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTTCACAGGCAAGAAGAC |
| SD | ACTCACTAGGAGATCAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11864A-A11 | VGB6 | C57BL/6NTac | |
| 11864A-F6 | VGB6 | C57BL/6NTac | |
| 11864A-F11 | VGB6 | C57BL/6NTac | |
