| Gene | Hsd3b7 |
| Chromosome | 7 |
| Deletion Start | 127,801,084 |
| Deletion End | 127,803,213 |
| Deletion Size | 2,130 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,801,189 | 127,801,207 | CGGGAGCTGCGTGTCTTTG |
| TUP | 127,801,209 | 127,801,233 | CCTGCACCTGAGTTCTTGGCTGGAG |
| TUR | 127,801,239 | 127,801,259 | CCACTCCAGTCACCTGCTTTC |
| TDF | 127,802,909 | 127,802,929 | TGGAGTTTCTGAGTCCCTGTG |
| TDP | 127,802,931 | 127,802,953 | TCTTCGACTGATAGGCGCCCACC |
| TDR | 127,802,954 | 127,802,972 | AGCCAGTAGGGCAGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGCCTGCCTTTTAGCCTG |
| SD | GTCTCACTACCTCTTCCTGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11865A-F8 | VGB6 | C57BL/6NTac | |
