11867   Vps37d     Status: ES cell colonies screened / QC positives

GeneVps37d
Chromosome5
Deletion Start135,077,608
Deletion End135,072,973
Deletion Size4,636
Genome Build38
MGI IDMGI:2159402
NCBI Gene ID194309
BAC ID290G21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,077,324135,077,343GGCTTGGCCCTCCTAGAAAC
TUP135,077,299135,077,323AGGCTCAAACAGCCGTCTTCAGGGC
TUR135,077,276135,077,296ACCTAGCAGAAGGCAGCACTG
TDF135,074,298135,074,317TCCGTGATTGCTTCCAGAAC
TDP135,074,274135,074,293TGGACTCCGCTCAGGACGGC
TDR135,074,198135,074,220CCCTGAGAAATGGGCCTTACTAC

PCR Primers

PrimerSequence
SUTGCTATGGCTCGGGAACAC
SDCCTCTGGAAGAAGCAGGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR68 bp++-
TDFTDR120 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11867A-A9VGB6C57BL/6NTac
11867A-B3VGB6C57BL/6NTac
11867A-B10VGB6C57BL/6NTac
11867A-C8VGB6C57BL/6NTac
11867A-D10VGB6C57BL/6NTac
11867A-F10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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