| Gene | Aanat |
| Chromosome | 11 |
| Deletion Start | 116,595,640 |
| Deletion End | 116,597,098 |
| Deletion Size | 1,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,596,162 | 116,596,181 | TGGATGAGATCCGGCACTTC |
| TUP | 116,596,184 | 116,596,208 | AACCCTGTGTCCAGAGCTGTCACTG |
| TUR | 116,596,244 | 116,596,261 | TCCCACAGCGAGCCAATG |
| TDF | 116,596,734 | 116,596,755 | GGAGAAACAGGTGTAGCAGAAG |
| TDP | 116,596,759 | 116,596,781 | CCCAGACATCTGTCCCTTGCCCA |
| TDR | 116,596,789 | 116,596,809 | CCTGTGTAGTGTCAGCGACTC |
PCR Primers
| Primer | Sequence |
| SU | GTTATGGTGCCCACAGTCTC |
| SD | GTCTTGCCTTTGTGCACAGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11868C-F1 | VGB6 | C57BL/6NTac | |
| 11868C-G4 | VGB6 | C57BL/6NTac | |
