| Gene | 1810027O10Rik |
| Chromosome | 11 |
| Deletion Start | 69,838,568 |
| Deletion End | 69,839,634 |
| Deletion Size | 1,067 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,838,802 | 69,838,825 | ACTACATCTGTTTCCACCAGTCAG |
| TUP | 69,838,832 | 69,838,858 | CCAGGGATGCAGTGTCTACTTAGGTGC |
| TUR | 69,838,870 | 69,838,894 | GAACTGTATTGGCTTAGACCCTCTC |
| TDF | 69,839,402 | 69,839,422 | TGCACCAGCTTCTACTACCAG |
| TDP | 69,839,425 | 69,839,448 | TCTGAGTGGAGACACCAGCATCCA |
| TDR | 69,839,477 | 69,839,497 | GGCAAGCCAGCCCAAGATTAG |
PCR Primers
| Primer | Sequence |
| SU | GTGACGCAAATCCTCTAGTC |
| SD | CTCATGTGCTCTTTAGGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11870A-E4 | VGB6 | C57BL/6NTac | |
| 11870C-B4 | VGB6 | C57BL/6NTac | |
| 11870C-E12 | VGB6 | C57BL/6NTac | |
