| Gene | Tbxa2r |
| Chromosome | 10 |
| Deletion Start | 81,332,482 |
| Deletion End | 81,334,732 |
| Deletion Size | 2,251 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,333,010 | 81,333,029 | GGCTCTTGGTGCTTCCTGAC |
| TUP | 81,333,030 | 81,333,051 | ACTCGGGACACAGCGCGGTGAC |
| TUR | 81,333,064 | 81,333,084 | GCCCAGGAGTGCGAATATGAG |
| TDF | 81,334,471 | 81,334,492 | CAGACTTTGTTGCAGACACCAC |
| TDP | 81,334,494 | 81,334,516 | TGTCATGAGCTTCTCTGGGCAGC |
| TDR | 81,334,543 | 81,334,562 | GCCACACGCAGGTAGATGAG |
PCR Primers
| Primer | Sequence |
| SU | CGCAGGACCAACAGTGATC |
| SD | TGTCTCAGACTCAGATGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11873A-D5 | VGB6 | C57BL/6NTac | |
| 11873A-D11 | VGB6 | C57BL/6NTac | |
| 11873A-F7 | VGB6 | C57BL/6NTac | |
