| Gene | 3110001D03Rik |
| Chromosome | 4 |
| Deletion Start | 75,277,941 |
| Deletion End | 75,277,395 |
| Deletion Size | 547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,278,078 | 75,278,097 | TGTCGGGCTCTACACTCTTC |
| TUP | 75,278,049 | 75,278,073 | CGGGCACCTACGTCTATTTGGTGGC |
| TUR | 75,278,017 | 75,278,038 | CAGGTGGGATTCCTTGCTTCAG |
| TDF | 75,277,896 | 75,277,916 | CCTCACGGCCCTGTAAGAAAG |
| TDP | 75,277,863 | 75,277,892 | ACCGAATGATCGACCTTTCTTCTTCAACCA |
| TDR | 75,277,823 | 75,277,843 | ACCCAGTGCTCTGCATAAAGC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGCTTAGAGATCCACAG |
| SD | ACAGAGAACCATGTTTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11879A-D3 | VGB6 | C57BL/6NTac | |
| 11879A-B9 | VGB6 | C57BL/6NTac | |
