| Gene | Eef1b2 |
| Chromosome | 1 |
| Deletion Start | 63,177,215 |
| Deletion End | 63,179,645 |
| Deletion Size | 2,431 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,177,960 | 63,177,982 | GTGAGCTGAATTTCTTTGTGACC |
| TUP | 63,177,985 | 63,178,012 | ACAAGGCCAAGAGACTGAAGTCCTCCAT |
| TUR | 63,178,016 | 63,178,039 | TCCATGTTCTACCTGTTCTGTGAG |
| TDF | 63,178,840 | 63,178,862 | GGAAGCAAAGAAGCTACGAGAAG |
| TDP | 63,178,863 | 63,178,889 | AACGCCTTGCACAGTATGAGTCCAAGA |
| TDR | 63,178,919 | 63,178,943 | GACAAGCTCCTGAAATCAACACTAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCGGAGTCCTTGGAGCG |
| SD | TCGAAGGCATCTAGTGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11880A-E11 | VGB6 | C57BL/6NTac | |
| 11880B-C12 | VGB6 | C57BL/6NTac | |
| 11880C-B2 | VGB6 | C57BL/6NTac | |
| 11880C-F2 | VGB6 | C57BL/6NTac | |
| 11880C-D10 | VGB6 | C57BL/6NTac | |
