| Gene | Morn3 |
| Chromosome | 5 |
| Deletion Start | 123,046,697 |
| Deletion End | 123,037,181 |
| Deletion Size | 9,517 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,046,692 | 123,046,713 | CCAGAAGAACGGACTGAGACAC |
| TUP | 123,046,666 | 123,046,691 | CAAGTGTTTGCTGTGAACGGCGATCA |
| TUR | 123,046,611 | 123,046,630 | GGCTCTCCCGCTTAGCTTAC |
| TDF | 123,037,754 | 123,037,772 | TGAAGGCTACTGGGTGGAC |
| TDP | 123,037,728 | 123,037,751 | CGTGGCCAAGTGTGGCACCATGAT |
| TDR | 123,037,707 | 123,037,724 | GCCTCATCACGGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCCATCACATCCCTGAC |
| SD | CAAACTCAAACCTGACTGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11883A-H5 | VGB6 | C57BL/6NTac | |
| 11883A-D9 | VGB6 | C57BL/6NTac | |
