| Gene | Golt1b |
| Chromosome | 6 |
| Deletion Start | 142,387,348 |
| Deletion End | 142,401,625 |
| Deletion Size | 14,278 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,387,846 | 142,387,863 | CTGGCCTTGCGCTCATTG |
| TUP | 142,387,864 | 142,387,889 | TGTCAGTGCTAGCCTTCCCACTTGTA |
| TUR | 142,387,917 | 142,387,940 | GCAGAGTCCTTCATGCTTGTAATC |
| TDF | 142,401,468 | 142,401,491 | CCAGCATCTAAAGGGCTTAGTCAG |
| TDP | 142,401,492 | 142,401,515 | TGCTGTGCGGTGTTTGAGTTCCCT |
| TDR | 142,401,546 | 142,401,570 | TGTTGCTTTCTCCAACTTTGTCTAC |
PCR Primers
| Primer | Sequence |
| SU | ACCACCACGAACCTGTCAG |
| SD | TGTACGAACGGCTGTTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11884A-B7 | VGB6 | C57BL/6NTac | |
| 11884A-E2 | VGB6 | C57BL/6NTac | |
| 11884A-F9 | VGB6 | C57BL/6NTac | |
| 11884A-G4 | VGB6 | C57BL/6NTac | |
