| Gene | Prdx4 |
| Chromosome | X |
| Deletion Start | 155,338,359 |
| Deletion End | 155,323,975 |
| Deletion Size | 14,385 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,338,230 | 155,338,249 | GCAAGGCTTGGAGAGTGATG |
| TUP | 155,338,209 | 155,338,229 | AACGGTTCCGGACCCGCGAAA |
| TUR | 155,338,181 | 155,338,200 | TGTCCACCAGCGTAGAAGTG |
| TDF | 155,324,902 | 155,324,924 | TGTGCTGTTAGCCAGTCTTAGTG |
| TDP | 155,324,869 | 155,324,897 | AAACCCATAATCCCAGTTATTTGGGAGGC |
| TDR | 155,324,833 | 155,324,854 | TTAGGCAGGCCATGAACTTTTG |
PCR Primers
| Primer | Sequence |
| SU | AGTCCTTCACAGGCACATAGG |
| SD | ATCTACCTTGATCAGTAAATATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11885B-A2 | VGB6 | C57BL/6NTac | |
| 11885B-C9 | VGB6 | C57BL/6NTac | |
| 11885B-G5 | VGB6 | C57BL/6NTac | |
| 11885C-C7 | VGB6 | C57BL/6NTac | |
| 11885C-F7 | VGB6 | C57BL/6NTac | |
| 11885C-H1 | VGB6 | C57BL/6NTac | |
