| Gene | Grcc10 |
| Chromosome | 6 |
| Deletion Start | 124,741,068 |
| Deletion End | 124,739,359 |
| Deletion Size | 1,710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,740,694 | 124,740,711 | TGCTTCCGTCTCGCAGTG |
| TUP | 124,740,665 | 124,740,690 | CTGGCGGAGGTGATTCAAGCGTTCTC |
| TUR | 124,740,611 | 124,740,629 | TCGTTGCACGCATTGTCTC |
| TDF | 124,739,421 | 124,739,441 | GTGTGGCTTGACTTGCTTCTG |
| TDP | 124,739,392 | 124,739,416 | CAGGTGTCCTTAAGTTTGCCCGCCT |
| TDR | 124,739,344 | 124,739,363 | CCTGACAGGCTGGCAATCTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTTCTCTGGTTTCCTAGG |
| SD | TGCCACCTTTGTGAGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11890A-A8 | VGB6 | C57BL/6NTac | |
| 11890A-C12 | VGB6 | C57BL/6NTac | |
| 11890A-G11 | VGB6 | C57BL/6NTac | |
