| Gene | Psmc4 |
| Chromosome | 7 |
| Deletion Start | 28,049,979 |
| Deletion End | 28,041,748 |
| Deletion Size | 8,232 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,049,291 | 28,049,313 | GTCAGGTACTGGAGACATAGGTG |
| TUP | 28,049,261 | 28,049,288 | TTGGTGGCGAAATAGGAAGAACGCTTTC |
| TUR | 28,049,222 | 28,049,244 | CTTGGTGTTGGAAGCAGAGAATC |
| TDF | 28,041,917 | 28,041,939 | CATTCCCATCCCTTCCTAGAGTG |
| TDP | 28,041,891 | 28,041,914 | ATGTTGGCTGTCCGTGAGAACCGC |
| TDR | 28,041,843 | 28,041,866 | GATCACGGTCTTGTATGCTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | AACAAGAGTTACTGCGCACG |
| SD | ATCTTCGAAGCCATTTGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11892A-B1 | VGB6 | C57BL/6NTac | |
| 11892D-E5 | VGB6 | C57BL/6NTac | |
| 11892C-A12 | VGB6 | C57BL/6NTac | |
| 11892C-B10 | VGB6 | C57BL/6NTac | |
