| Gene | Dbndd2 |
| Chromosome | 2 |
| Deletion Start | 164,488,253 |
| Deletion End | 164,490,365 |
| Deletion Size | 2,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,488,705 | 164,488,727 | GGTATGTCCTCTGCTTTGGAATC |
| TUP | 164,488,728 | 164,488,753 | TGGGTACCATTTGGCCAGAGCTGGAT |
| TUR | 164,488,776 | 164,488,795 | CCCTGGCTCAGACCTACAAC |
| TDF | 164,489,572 | 164,489,594 | TCAGTCACATAGGCAGGAAGTAG |
| TDP | 164,489,597 | 164,489,623 | TGCTGATATTCAAACCCTCTGCCTGGC |
| TDR | 164,489,626 | 164,489,649 | AACTTGGTAAGCATTCAGGGTCTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCAGTTAGCCAGCATTGG |
| SD | CTAAGAAAGCAGCTGCGTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11897A-A4 | VGB6 | C57BL/6NTac | |
| 11897B-F5 | VGB6 | C57BL/6NTac | |
