| Gene | Tprkb |
| Chromosome | 6 |
| Deletion Start | 85,924,385 |
| Deletion End | 85,929,573 |
| Deletion Size | 5,189 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,925,075 | 85,925,097 | CTCCTCTATCAAGGCCACATCTC |
| TUP | 85,925,100 | 85,925,129 | ATGATGGCTCTTCTTAGTCCTCAACCTGAC |
| TUR | 85,925,155 | 85,925,174 | CAGGTATGCCCAGCCATTTG |
| TDF | 85,928,687 | 85,928,710 | GCCGTAGTGAAATGATAAACCACA |
| TDP | 85,928,729 | 85,928,756 | AGTAGCACGTGGACACAGCATTCATAGA |
| TDR | 85,928,794 | 85,928,816 | AACGTCCCAATCCTCTCTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTAGAGAAGAAACAGGGAAG |
| SD | GTGTGAATGAAAAGAGGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11899B-B2 | VGB6 | C57BL/6NTac | |
| 11899B-H1 | VGB6 | C57BL/6NTac | |
| 11899B-D4 | VGB6 | C57BL/6NTac | |
| 11899B-A5 | VGB6 | C57BL/6NTac | |
