| Gene | Ccdc120 |
| Chromosome | X |
| Deletion Start | 7,739,861 |
| Deletion End | 7,734,796 |
| Deletion Size | 5,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,737,616 | 7,737,634 | TGCTCAGTGACCCAGAGAC |
| TUP | 7,737,592 | 7,737,614 | TGGCCCGCTGGAGAACTATACGC |
| TUR | 7,737,521 | 7,737,540 | AGCTTGGCCCTGCTCAATAC |
| TDF | 7,734,117 | 7,734,138 | TGTCTGACTCTGCCCTTGTTAC |
| TDP | 7,734,092 | 7,734,114 | CACCTCTGCTCTCCAGACCGGAA |
| TDR | 7,734,057 | 7,734,076 | TCCTGACTACCGGACCACTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCCACTTACTGTGTTAG |
| SD | AGCTTGGAAGAGAAAGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11902A-A3 | VGB6 | C57BL/6NTac | |
| 11902A-A6 | VGB6 | C57BL/6NTac | |
| 11902A-C7 | VGB6 | C57BL/6NTac | |
| 11902A-E3 | VGB6 | C57BL/6NTac | |
| 11902A-E10 | VGB6 | C57BL/6NTac | |
| 11902A-F3 | VGB6 | C57BL/6NTac | |
