| Gene | Yrdc |
| Chromosome | 4 |
| Deletion Start | 124,850,829 |
| Deletion End | 124,854,571 |
| Deletion Size | 3,743 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,851,338 | 124,851,359 | GTCGGTCGAGAAGCTGAGTTAG |
| TUP | 124,851,365 | 124,851,387 | CATGGCACTCTTCTCCCGGTGCC |
| TUR | 124,851,421 | 124,851,445 | CGTTTAATCATCTGGAAGGCGTTAC |
| TDF | 124,853,970 | 124,853,993 | GTTGACTTATCTGTGCCTGGAAAG |
| TDP | 124,853,994 | 124,854,017 | TTTGGCATTATTCGCCCAGGCTGG |
| TDR | 124,854,043 | 124,854,064 | TTCTCCAACCTCCCTCACATTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGTCCGCTAAGCCGAG |
| SD | ACTGCATTAGTGCTTCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11903A-D11 | VGB6 | C57BL/6NTac | |
| 11903A-G8 | VGB6 | C57BL/6NTac | |
| 11903A-H9 | VGB6 | C57BL/6NTac | |
