| Gene | Cops7a |
| Chromosome | 6 |
| Deletion Start | 124,964,414 |
| Deletion End | 124,959,072 |
| Deletion Size | 5,343 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,964,787 | 124,964,805 | GGCCACACTCATCCACCAG |
| TUP | 124,964,765 | 124,964,785 | TGCTGGAGGCCCCTGGTGTCT |
| TUR | 124,964,743 | 124,964,762 | TCCAGCAGTTCCCCAAACAC |
| TDF | 124,960,216 | 124,960,234 | CGCCTCCTGCTGACTTCTC |
| TDP | 124,960,193 | 124,960,214 | TCTGCCTGCTTCCCACAGGTGC |
| TDR | 124,960,157 | 124,960,177 | CTCTTCGATGCCCGACAACAC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTTCATCAGTCTTGGAAC |
| SD | TAGGACTGCCAACCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11905A-D12 | VGB6 | C57BL/6NTac | |
| 11905A-E7 | VGB6 | C57BL/6NTac | |
| 11905A-E8 | VGB6 | C57BL/6NTac | |
