| Gene | Mutyh |
| Chromosome | 4 |
| Deletion Start | 116,814,328 |
| Deletion End | 116,819,434 |
| Deletion Size | 5,107 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,814,441 | 116,814,459 | GGAGCTTGTCCCTCACCAG |
| TUP | 116,814,463 | 116,814,485 | CAAGGAGCTGGTTTCACTGGCCA |
| TUR | 116,814,496 | 116,814,518 | GGTTCACAGCAAGCCTGAATCTG |
| TDF | 116,818,936 | 116,818,958 | CTTAACCACTGACCCATCCATTC |
| TDP | 116,818,963 | 116,818,987 | CCTCATTGTGCAGCTGAAGATGGCC |
| TDR | 116,818,990 | 116,819,014 | CATGGTGAAGGAGGATTACAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGTGAGACCCTACTTCAGAAGAC |
| SD | GGTGCAGACTAAGAGGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11906A-C4 | VGB6 | C57BL/6NTac | |
| 11906A-G11 | VGB6 | C57BL/6NTac | |
| 11906A-C7 | VGB6 | C57BL/6NTac | |
| 11906A-F2 | VGB6 | C57BL/6NTac | |
