| Gene | Trh |
| Chromosome | 6 |
| Deletion Start | 92,244,153 |
| Deletion End | 92,242,834 |
| Deletion Size | 1,320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,243,828 | 92,243,850 | GGCTTTGATCTTCGTGCTAACTG |
| TUP | 92,243,798 | 92,243,822 | CCCAAATCCTGCGCCTTGCTGGAAG |
| TUR | 92,243,757 | 92,243,775 | GCCTGGAAGGTCAGGAGTC |
| TDF | 92,243,166 | 92,243,185 | GCAAGCAAGGGTTCCTGATG |
| TDP | 92,243,145 | 92,243,165 | ACGGGACCAGGCTCTCCCACA |
| TDR | 92,243,062 | 92,243,084 | GCTTCAACGTCTTCCTCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGTCAGATGCAGCGCTGAC |
| SD | GTCTTGGCCAGTGTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11909A-D8 | VGB6 | C57BL/6NTac | |
| 11909A-D12 | VGB6 | C57BL/6NTac | |
| 11909A-E4 | VGB6 | C57BL/6NTac | |
| 11909A-G5 | VGB6 | C57BL/6NTac | |
| 11909A-E12 | VGB6 | C57BL/6NTac | |
