| Gene | AI464131 |
| Chromosome | 4 |
| Deletion Start | 41,501,187 |
| Deletion End | 41,499,037 |
| Deletion Size | 2,151 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,499,473 | 41,499,495 | CCAAACCCAAGCCCACTAAAGAG |
| TUP | 41,499,450 | 41,499,472 | CTGAGACCTCTGCTGTGCTCCGC |
| TUR | 41,499,401 | 41,499,418 | GTAGCACCAGGCCACAAC |
| TDF | 41,498,153 | 41,498,171 | CAGGCGGTACACCGAGATG |
| TDP | 41,498,125 | 41,498,148 | AGCCCTTCTTCTCGCTAGCCGAGG |
| TDR | 41,498,081 | 41,498,102 | ACGGAAGAAGCAGGAGATGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCATTCACAGAGTTCTCACC |
| SD | AAGAGGCTTTCTGCAGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11910A-C12 | VGB6 | C57BL/6NTac | |
| 11910A-E6 | VGB6 | C57BL/6NTac | |
| 11910A-F6 | VGB6 | C57BL/6NTac | |
