| Gene | Ing2 |
| Chromosome | 8 |
| Deletion Start | 47,673,800 |
| Deletion End | 47,667,659 |
| Deletion Size | 6,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,674,543 | 47,674,562 | CCCACGACATGCAGAGGAAC |
| TUP | 47,674,512 | 47,674,535 | TGCTGCGGGAGCTGGACAACAAAT |
| TUR | 47,674,482 | 47,674,502 | GTCACCCGAAAGAAGGGAAAG |
| TDF | 47,669,098 | 47,669,116 | TGAGCGAGCCTCAGACAAG |
| TDP | 47,669,072 | 47,669,097 | TCGAAGATGGATTCCAGTCAACCGGA |
| TDR | 47,669,040 | 47,669,060 | TCCTCTGTCTTCGAGGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTCGCTCTCCTAGAGA |
| SD | TGACACAGAAATCCAACAATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11911A-B12 | VGB6 | C57BL/6NTac | |
| 11911A-E10 | VGB6 | C57BL/6NTac | |
