11912   Pabpn1l     Status: ES cell colonies screened / QC positives

GenePabpn1l
Chromosome8
Deletion Start122,622,500
Deletion End122,619,533
Deletion Size2,968
Genome Build38
MGI IDMGI:2685954
NCBI Gene ID382035
BAC ID242J12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,621,881122,621,902CCACCTGTGTTCAGTGGAAGAG
TUP122,621,856122,621,880TACTCGGCATGGGCTGCATATGAGG
TUR122,621,806122,621,824GCGGTCACACTGTGGATTG
TDF122,620,276122,620,295ATGGACGGAGCCGGTAAGTG
TDP122,620,248122,620,273CCCAAGTGGGACTCTGAGTCTGGAGA
TDR122,620,204122,620,223ACAGCCAGCATAAGGGTCAG

PCR Primers

PrimerSequence
SUTGAGTGTTTCAGGGATGCAG
SDAATGCTAACCTGTATCTGAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR97 bp++-
TDFTDR92 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11912A-B1VGB6C57BL/6NTac
11912A-C2VGB6C57BL/6NTac
11912A-G4VGB6C57BL/6NTac
11912A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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