| Gene | Prr14 |
| Chromosome | 7 |
| Deletion Start | 127,471,958 |
| Deletion End | 127,476,661 |
| Deletion Size | 4,704 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,472,368 | 127,472,388 | GCAGCCTCCATAATGCAAGAC |
| TUP | 127,472,399 | 127,472,425 | TGGGTAATTCATGTGCCTGTCCAGTCA |
| TUR | 127,472,430 | 127,472,451 | TAGTAGGATGTGGTTGGGTGAG |
| TDF | 127,475,811 | 127,475,834 | TGATCCAGAGACCAAGGTAATGAG |
| TDP | 127,475,837 | 127,475,864 | AGTATAAAGACAGGAGCCAGCCAAGGCC |
| TDR | 127,475,876 | 127,475,896 | TGGGCAGAGGGTGAAAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTAACAGGGACAAAGGTCC |
| SD | TTTCTAGTCCTTTGGTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11914A-B1 | VGB6 | C57BL/6NTac | |
| 11914A-B9 | VGB6 | C57BL/6NTac | |
| 11914A-G8 | VGB6 | C57BL/6NTac | |
| 11914A-E8 | VGB6 | C57BL/6NTac | |
| 11914A-F2 | VGB6 | C57BL/6NTac | |
| 11914A-E4 | VGB6 | C57BL/6NTac | |
