| Gene | Atp5sl |
| Chromosome | 7 |
| Deletion Start | 25,620,453 |
| Deletion End | 25,624,948 |
| Deletion Size | 4,496 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,620,863 | 25,620,884 | CAACCTCAGTCCTTTCCCTCTC |
| TUP | 25,620,885 | 25,620,907 | CCCTTCTCCAGGTCCTGCACCTG |
| TUR | 25,620,917 | 25,620,936 | CTGGCTCTGCCATTCCATTC |
| TDF | 25,624,007 | 25,624,028 | CCTGCCTTCTTACTCACCAGTG |
| TDP | 25,624,031 | 25,624,052 | CCTGAAGGAGCTGCGGTCCCTG |
| TDR | 25,624,098 | 25,624,119 | GTGAGCCAGCCAATAGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | AATTTGGAGGTCCGAGGAAG |
| SD | TACTTTGGATTCTCTCCGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11915A-H2 | VGB6 | C57BL/6NTac | |
| 11915B-B10 | VGB6 | C57BL/6NTac | |
