| Gene | Gjb4 |
| Chromosome | 4 |
| Deletion Start | 127,353,845 |
| Deletion End | 127,353,024 |
| Deletion Size | 822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,351,876 | 127,351,896 | TCACCTGTCCTTCCCTGTTAG |
| TUP | 127,351,844 | 127,351,870 | ATGCATGTGGCCTATCGTGAAGAGCGA |
| TUR | 127,351,817 | 127,351,838 | CCCATGTTTGAGGCGATGTTTC |
| TDF | 127,351,449 | 127,351,469 | GAGGCACCAACTGCCAGATAC |
| TDP | 127,351,422 | 127,351,447 | TGCCCACCGTATGTGATCTCCAAAGG |
| TDR | 127,351,374 | 127,351,393 | GCCATCCCGGCCTTTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | AATGTCCTCATGGTTGTTGTG |
| SD | TGGAGTTGAGGTCCTCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11918A-A9 | VGB6 | C57BL/6NTac | |
| 11918A-H7 | VGB6 | C57BL/6NTac | |
| 11918A-F7 | VGB6 | C57BL/6NTac | |
| 11918A-F2 | VGB6 | C57BL/6NTac | |
