| Gene | Taf9b |
| Chromosome | X |
| Deletion Start | 106,219,626 |
| Deletion End | 106,208,445 |
| Deletion Size | 11,182 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,219,005 | 106,219,022 | CATCGCGCATTCCGTCTG |
| TUP | 106,218,969 | 106,218,998 | TGTCTTTCTTTGAATCCACAGGTGATGGCA |
| TUR | 106,218,924 | 106,218,945 | AACCCTTGGCTCATACTCTGTG |
| TDF | 106,208,563 | 106,208,582 | CCAGCATGGTTTCCTCACAG |
| TDP | 106,208,537 | 106,208,560 | CACAGCCACCGACTCAAACCCACT |
| TDR | 106,208,501 | 106,208,523 | GTGTCATTGTCATCGTCGTCATC |
PCR Primers
| Primer | Sequence |
| SU | AATTCCAGGAAACTCCGAGG |
| SD | TAAGGCGATGAGAGGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11919B-A3 | VGB6 | C57BL/6NTac | |
| 11919B-G10 | VGB6 | C57BL/6NTac | |
