| Gene | Cbx8 |
| Chromosome | 11 |
| Deletion Start | 119,040,711 |
| Deletion End | 119,038,504 |
| Deletion Size | 2,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,040,101 | 119,040,118 | GCCTCCTTGCAGCCTTTG |
| TUP | 119,040,072 | 119,040,100 | AGGAAAGGTACAGACCCTTAGCCTAGCCA |
| TUR | 119,040,049 | 119,040,071 | CAGCGTGTATGGGTTCTAGTGAG |
| TDF | 119,039,058 | 119,039,078 | GACCTGGTGCAATATGGTGTG |
| TDP | 119,039,034 | 119,039,056 | CCAGTCCTAGCTCAGCAGAGGCC |
| TDR | 119,038,997 | 119,039,015 | CCCTGGCTGGAAAGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTGGAGTGGACAGAGAC |
| SD | CTAATAGAAAATAGAACTCTAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11920A-H1 | VGB6 | C57BL/6NTac | |
| 11920A-F7 | VGB6 | C57BL/6NTac | |
| 11920A-A5 | VGB6 | C57BL/6NTac | |
| 11920A-B10 | VGB6 | C57BL/6NTac | |
