| Gene | Uqcrh |
| Chromosome | 4 |
| Deletion Start | 116,074,860 |
| Deletion End | 116,067,033 |
| Deletion Size | 7,828 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,074,105 | 116,074,127 | GCAAGACAATACAACTGGGACTC |
| TUP | 116,074,079 | 116,074,104 | CAATGCAGACGTCCAGGCTTGAGAGC |
| TUR | 116,074,035 | 116,074,056 | TGCACAGGAAATGGAAGCAAAG |
| TDF | 116,067,814 | 116,067,834 | TAGGGCCTGCGTATGGATAAC |
| TDP | 116,067,782 | 116,067,809 | TGAAGAATGAGGGTCAAGACAGGAGCTC |
| TDR | 116,067,761 | 116,067,779 | TCCTTCCTGGCCTGAACAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAAGTGACCGAAAGGATG |
| SD | AAGAGCAAGTTTGCAGATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11922A-A12 | VGB6 | C57BL/6NTac | |
| 11922A-C9 | VGB6 | C57BL/6NTac | |
| 11922A-F10 | VGB6 | C57BL/6NTac | |
| 11922A-F7 | VGB6 | C57BL/6NTac | |
| 11922A-E3 | VGB6 | C57BL/6NTac | |
| 11922A-H6 | VGB6 | C57BL/6NTac | |
