| Gene | Med29 |
| Chromosome | 7 |
| Deletion Start | 28,392,670 |
| Deletion End | 28,386,770 |
| Deletion Size | 5,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,392,315 | 28,392,338 | GACAGACTAGCTGTGTTGTGTTTG |
| TUP | 28,392,286 | 28,392,309 | AACCAAAGTGAAGCTCCGGTCAGC |
| TUR | 28,392,266 | 28,392,283 | AAGAACGCGCCACCACTA |
| TDF | 28,387,631 | 28,387,651 | CCTACAGACAGACCACTGACC |
| TDP | 28,387,601 | 28,387,630 | AGCTCAGATATGTGCACTGATGATCCCAGC |
| TDR | 28,387,576 | 28,387,597 | CCTCTACTGCTTCATCCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AGTCCTTCACAGTGATTGGC |
| SD | CTAAAGGGCTCAGTTAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11924A-B10 | VGB6 | C57BL/6NTac | |
| 11924A-F2 | VGB6 | C57BL/6NTac | |
