| Gene | Mrpl53 |
| Chromosome | 6 |
| Deletion Start | 83,109,115 |
| Deletion End | 83,109,675 |
| Deletion Size | 561 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,109,150 | 83,109,171 | CAAGCTGGTTCGAGTTCAGTTC |
| TUP | 83,109,172 | 83,109,194 | TGCCCGTTTGAGAAGAACGTGGA |
| TUR | 83,109,195 | 83,109,212 | AACCCGTACCTCGTGGAC |
| TDF | 83,109,521 | 83,109,544 | GGAGATGGGTATCGGCTGATTATG |
| TDP | 83,109,547 | 83,109,568 | CGGTGCCCACCTCACCACTCAG |
| TDR | 83,109,593 | 83,109,611 | CGTTCCTGTCCCGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TAGATCTCGCAGGCAGGAC |
| SD | ATGTACTTGGTTGACAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11927C-H2 | VGB6 | C57BL/6NTac | |
| 11927C-F5 | VGB6 | C57BL/6NTac | |
