| Gene | Rpl38 |
| Chromosome | 11 |
| Deletion Start | 114,668,815 |
| Deletion End | 114,672,388 |
| Deletion Size | 3,574 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,669,340 | 114,669,358 | GGCCGAGGCAAGAGAACTG |
| TUP | 114,669,359 | 114,669,385 | AGCATCCAACGCTATCCTTGGCCTACA |
| TUR | 114,669,400 | 114,669,419 | GAACCCAGCGCAGGAAGAAG |
| TDF | 114,671,900 | 114,671,918 | GGAGCCACTTTGTGGTGAC |
| TDP | 114,671,919 | 114,671,944 | TGTGAGGTGTCTGCCATGACTGAGTT |
| TDR | 114,671,967 | 114,671,986 | GGGACCAAGGAAGCCATTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCGTACTCAGTTTCCTCTG |
| SD | CAGTCCATGGTAAGGCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11928B-D10 | VGB6 | C57BL/6NTac | |
| 11928B-H4 | VGB6 | C57BL/6NTac | |
| 11928B-H8 | VGB6 | C57BL/6NTac | |
| 11928C-G4 | VGB6 | C57BL/6NTac | |
| 11928C-G5 | VGB6 | C57BL/6NTac | |
